Defi ciencies of Factor XI (FXID) and Uridine Monophosphate Synthase (DUMPS) are autosomal recessive inherited diseases in Holstein cattle breed. The aim of this study was to investigate whether FXI defi ciency and DUMPS alleles exist in Holstein cattle reared in Antalya region Turkey or not. 504 Holstein cows were used for this study. PCR (Polymerase Chain Reaction) was used for determination ...

The aim of this study was to identify the deficiency of uridine monophosphate synthase (DUMPS) and the complex vertebral malformation (CVM) in Iranian Holstein bulls. A total of 144 blood samples were prepared of Holstein bulls in Abbas Abad Animal Breeding Center and Ferdowsi University of Mashhad's Dairy Farm in Khorasan state of Iran. Genomic PCR-RFLP protocol was performed to amplify the po...

هدیکچ فده و هقباس : نیئتورپ نایب ا یکی نارادناتسپ ریش رد یجراخ یاه هار ز یعیـسو دربراک جیردت هب هک تسا ییاهراک هدروآرف هوبنا دیلوت رد یم بیکرتون ییوراد یاه هب ،دبای هدروآرف هژیو تسد هک ییاه شیب شوخ سـپ تارـییغت نیرت زا همجرت یا یم دنوش . شوم داجیا قیقحت نیا زا فده سنارت یاه سنارت دجاو هک دوب یکینژ نایب نژ هدننک یسلک نینوـت رد دیربیه دنشاب شوم یریش ددغ . شور و داوم اه : یدنفـسوگ نیلوبلگوتک...

The polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP) experiment has the characteristics of low-cost, rapidity, simplicity, convenience, high sensitivity and specificity; thus, many small medium laboratories use it to perform all kinds single nucleotide polymorphisms (SNPs) genotyping works, as a molecular biotechnology for disease-related analysis. However, lack ava...

Among the various hereditary diseases that have been widely studied in dairy cattle, bovine leukocyte adhesion deficiency (BLAD), deficiency of uridine-5-monophosphate synthase (DUMPS), and complex vertebral malformation (CVM) are noteworthy because of their high impact on overall herd productivity as a consequence of increased calf mortality. The aim of this study was to verify the frequency o...

Calreticulin (CALR) mutations are included in the WHO diagnostic criteria of Myeloproliferative Neoplasm (MPN). Presence CALR mutation, aids their diagnosis by excluding reactive causes. There several molecular techniques to detect mutations; however, these require advanced equipment and technical expertise. An in-house “polymerase chain reaction-restriction fragment length polymorphism (PCR- R...

شور و داوم اه : ،یبرجت همین قیقحت نیا رد 28 نیماتیو ،نیرمت ،لرتنک هورگ راهچ هب یفداصت روط هب و باختنا قاچ رتخد C یبیکرت و دندش میسقت . نیرمت تدش اب يزاوه 50 ات 70 ،رثکادح بلق نابرض دصرد 3 تدم هب و هتفه رد هسلج 8 هتفه دش ماجنا . هورگ ياه نیماتیو C و صرق یبیکرت 500 یلیم نیماتیو یمرگ C ار 3 فرصم هتفه رد راب دندومن . هنومن و لبق اتشان ینوخ ياه 48 هلخادم نیرخآ زا سپ تعاس اه عم...